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Primary Antibodies  >  Cytokines & Growth Factor Antibodies
 

Estrogen Receptor Antibody

Rabbit Monoclonal SP1 IgG
Code

900-C01-B35
Size

100 µL
Price

$309.00
Availability

Ships next business day.
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Rockland's Anti-Estrogen Receptor (ER) Monoclonal Antibody (Rabbit) was used to detect ER in human breast tumor tissue. Tissue was formalin-fixed and paraffin embedded.  Staining requires boiling of sections in 10 mM citrate buffer pH 6.0 for 10 min followed by cooling at RT for 20 min.  The primary antibody was diluted 1:200 and reacted with tissue for 30 min at RT.
Western Blot anti-Estrogen Receptor (ER) antibody
Shipping & Handling Fees Apply.

Shipping:
$70.00 to United States
Handling:
$20.00
Synonyms: ER estradiol receptor ER-alpha nuclear receptor subfamily 3 group A member 1

Estrogen Receptor Antibody Properties

Description
Anti-ESTROGEN RECEPTOR (RABBIT) Monoclonal Antibody - 900-C01-B35
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Monoclonal SP1 IgG
Application
ELISA : 1:1,000 - 1:5,000
Western Blot : 1:500 - 1:3,000
Immunohistochemistry: 1:200
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Format
CCS
Label
Concentration
N/A
Buffer
None
Preservative
0.1% (w/v) Sodium Azide
Stabilizer
None
GeneName
ESR1
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Lot#  

Estrogen Receptor Antibody Description

Background
The ESR1 gene encodes an estrogen receptor (also known as ER, estradiol receptor, ER-alpha and nuclear receptor subfamily 3 group A member 1), which is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters.
Immunogen
This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to the carboxy terminal region of human Estrogen Receptor protein. The hybridoma was produced by fusing New Zealand White rabbit splenocytes and myeloma cells using conventional technology.
Immunogen Type
Peptide
Storage Condition
Store vial at 2-8° C prior to opening. Do not freeze. Dilute only prior to immediate use. 
Application Note
This monoclonal antibody is suitable for ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 67 kDa in size corresponding to Estrogen Receptor protein by western blotting in the appropriate cell lysate or extract. Estrogen Receptor is found in human breast carcinoma tissue as a nuclear protein. For immunohistochemistry, samples should be formalin fixed and paraffin embedded. Deparaffinize slides using xylene or xylene alternatives and graded alcohols. Staining requires boiling of sections in 10 mM citrate buffer pH 6.0 for 10 min followed by cooling at RT for 20 min.
Purity/Specifity
This product is clarified tissue culture supernate. The antibody is specific for human and hamster Estrogen Receptor protein. Cross-reactivity with Estrogen Receptor protein from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Greene G.L., Gilna P., Waterfield M., Baker A., Hort Y., Shine J. (1986) Sequence and expression of human estrogen receptor complementary DNA. Science 231:1150-1154. Green S., Walter P., Kumar V., Krust A., Bornert J.-M., Argos P., Chambon P. (1986) Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-A. Nature 320:134-139. Pink J.J., Wu S.Q., Wolf D.M., Bilimoria M.M., Jordan V.C. (1996) A novel 80 kDa human estrogen receptor containing a duplication of exons 6 and 7. Nucleic Acids Res. 24:962-969 Schubert E.L., Lee M.K., Newman B., King M.C. (1999) Single nucleotide polymorphisms (SNPs) in the estrogen receptor gene and breast cancer susceptibility. J. Steroid Biochem. Mol. Biol. 71:21-27.
Specific Reference
Cano G, Milanezi F, Leitão D, Ricardo S, Brito MJ, Schmitt FC. (2003) Estimation of Hormone Receptor Status in Fine-Needle Aspirates and Paraffin-embedded Sections from Breast Cancer Using theNovel Rabbit Monoclonal antibodies SP1 and SP2. Diaqn Cytopathol 4:207-11. Huang Z, Zhu W, Szekeres G, Xia H. (2005) Development of new rabbit monoclonal antibody to estrogen receptor (clone SP1): Immunohistochemical assessment on formalin-fixed, paraffin-embedded tissue sections. Appl Immunohistochem Mol Morphol. 1:91-5.
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
900-C01-B36

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry