The ESR1 gene encodes an estrogen receptor (also known as ER, estradiol receptor, ER-alpha and nuclear receptor subfamily 3 group A member 1), which is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters.
This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to the carboxy terminal region of human Estrogen Receptor protein. The hybridoma was produced by fusing New Zealand White rabbit splenocytes and myeloma cells using conventional technology.