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Custom Products  >  Custom Laboratory Services
 

IgY Purification

Code

CUST28
Size

1 Each
Price

Price On Request
Availability

Ships within 2 weeks.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
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Synonyms: Immunoglobulin Y, Egg, Bird, Chicken, Lizard, Lungfish Immunoglobulin, Chicken IgG, Egg Yolk IgG, and 7S-IgG.

IgY Purification Properties

Description
IgY PURIFICATION (up to 6 eggs) - CUST28
Application
ELISA : User Optimized
ChIP : User Optimized
Neutralization : User Optimized
Flow Cytometry : User Optimized
Western Blot : User Optimized
Immunohistochemistry: User Optimized
ImmunoPrecipitation: User Optimized
Shipping condition
Wet Ice
Label
Download CofA
Lot#  

IgY Purification Description

Background
Supply up to 6 eggs for a multi-step purification of IgY from the yolk resulting in >95% purity of IgY.
Application Note
Immunoglobulin Y in the chicken is functionally equivalent to Immunoglobulin G (IgG). It is composed of two light and two heavy chains. the difference is that the heavy chains of IgY are larger than those in IgG. The light chains in IgY are smaller than the light chains in IgG. The molar mass of IgY is about 167,000 amu. Its steric flexibility is somewhat less than that of IgG. IgY is partially comparable to Immunoglobulin E (IgE), as well as to IgG, in functionality. In contrast to IgG, however, IgY does not bind to Protein A or G, or to cellular Fc receptors. In addition, IgY does not activate the complement system.
Purity/Specifity
IgY cross-reacts much less with mammalian protein than does IgG. In addition, conserved mammalian proteins often are more immunogenic in birds than in mammals. The only immunoglobulin found in chicken eggs is IgY, from amongst all the other immunoglobulins that are generated during the immune response. There is no contamination from Immunoglobulin A (IgA) or Immunoglobulin M (IgM). .
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Rüdiger Schade, Irene Behn, Michael Erhard: Chicken Egg Yolk Antibodies, Production and Application. Springer-Verlag, Berlin 2001, ISBN 3-540-66679-6. Schade R, Hlinak A, Marburger A, Henklein P, Morgenstren R, Blankenstein P, Gerl M, Zott A, Pfister C, Erhard M (1997) Advantages of using egg yolk antibodies in the life sciences: The results of five studies. ATLA 25: 555-586.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-50

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry