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CXCR4 Antibody

Rabbit Polyclonal
NCI Collaboration
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Number:
200-401-G92
Size:
100 µg
Price:
$309.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships within 2 weeks.

CXCR4 Antibody Properties

Description
Anti-CXCR4 (RABBIT) Antibody - 200-401-G92
Host
Rabbit
Target Species
Human
Known Cross Reactivity
Human, Mouse
Clonality
Polyclonal
Application
Western Blot : 0.5-1 ug/mL
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
IgG
Label
Concentration
1 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
GeneName
CXCR4
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CXCR4 Antibody Description

Background
CXCR4 antibody detects a G coupled -protein receptor. Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2. The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites. Anti-CXCR4 antibodies are ideal for investigators involved in Cytokines and Growth Factors or Infectious Disease reasearch.
Synonyms
CXCR4 (NT), CXCR4, C-X-C chemokine receptor type 4, FB22, Fusin, HM89, LCR1, Leukocyte-derived seven transmembrane domain receptor, NPYRL, Stromal cell-derived factor 1 receptor, SDF-1 receptor
Immunogen
CXCR4 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a peptide corresponding to an internal sequence in the second extracellular loop (EL) of human CXCR4.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-CXCR4 Antibody is suitable for use in E, WB. Expect a band approximately ~39.7 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
Anti-CXCR4 Antibody was DEAE purified. A BLAST analysis was used to suggest cross-reactivity with CXCR4 with Human and Mouse based on 100% homology with the immunizing sequence. Cross-reactivity with CXCR4 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Dimitrov DS. Cell 1997;91:721-730 Feng Y et al. Science 1996;272:872-7 Berson JF et al. J Virol 1996;70:6288-95 Doranz BJ et al. Cell 1996;85:1149-1158
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, chicken
Catalog Number
100-401-401

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
mouse, human
Catalog Number
100-401-862

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry