AccuSignal ELISA Kits


AccuSignal ELISA Kits



Rockland offers a wide variety of ELISA kits in sandwich assay formats. This extensive portfolio includes over 800 complete, ready to use ELISA kits for detection of specific growth factors, cytokines, chemokines, hormones, glycoproteins, and neurotrophic factors that are common targets of research interest. Each enzyme linked immunosorbent assay is rigorously tested to ensure high precision, accuracy, sensitivity, and specificity. Each ELISA kit is optimized for accurate quantitation of targets in various sample types, including serum, plasma, saliva, cell lysates, and cell culture supernatants. 

 

 
AccuSignal ELISA Kit Categories
Growth Factor specific Cytokine & Chemokine specific
Hormone specific Glycoprotein specific
Human IgG & IgM specific DNA Damage & Nitrotyrosine specific

 

ELISA Kit Features
Sensitive and robust "sandwich" assay format Highly specific capture and detection antibody combination
Consistent performance Validated on serum, plasma, cell culture supernatant, or cell lysate samples
Ready-to-use, convenient assay Detailed instruction protocols


Elisa Kits


Components

  • Antibody-coated 96-well plate
  • Target Protein Standard
  • Detection antibody (typically biotinylated)
  • Detection reagent (usually Avidin-Biotin-Peroxidase complex)
  • Diluent buffers
  • Wash buffers
  • Substrate Solution
  • Stop solutions
  • Adhesive covers


Advantages

  • High specificity- Measures only an antigen of interest. Since two antibodies are used, the antigen is precisely captured and detected.
  • Sensitivity- Measures proteins at the pg/ml level.
  • Suitable for complex samples- Antigen does not need to be purified prior to analysis.
  • Quality Antibodies and Reagents- Optimized for sensitive, accurate, and outstanding performance.
  • Precision & Reproducibility- Repeated measurements show the consistent results.

 


Basics of ELISA Immunoassays

 

ELISA (Enzyme-linked immunosorbent assay) is a simple, cost-effective technique performed on serum, plasma, cell lysates, and cell supernatant for detecting and quantitating antigens such as peptides, proteins, antibodies, and hormones in a sample. 

 

The standard ELISA formats include a direct and indirect detection methods. The direct detection method can be performed with an antigen that is directly immobilized on a multi-well assay plate and is detected by a labeled primary antibody that reacts directly with the antigen.

 

In an indirect detection method, the primary antibody used to detect the antigen is not conjugated to an enzyme. Instead, an enzyme-conjugated secondary antibody that has specificity for the primary antibody is used to detect the primary antibody. 

 

The most powerful ELISA format is the sandwich assay. The sandwich ELISA quantifies antigen bound between two primary antibodies (i.e. capture antibody and the detection antibody). Sandwich ELISAs employ multi-well microtiter plates, pre-coated with capture antibodies, to bind the antigen of interest. The antigen to be measured must contain at least two epitopes capable of binding to antibody. Either monoclonal or polyclonal antibodies can be used as the capture and detection antibodies in Sandwich ELISA systems. The bound antigen is then detected with a subsequent detection antibody that is also specific to the antigen. The detection antibody can either be bound by a secondary antibody-which is typically labeled with an enzyme, or the detection antibody itself is enzyme-conjugated. When chromogenic substrate is added to the assay to change color, samples with high antigen concentration generate a signal that is directly proportional to the amount of antigen in the sample. This correlation can then be used to extrapolate the concentration of antigen in an unknown sample from a standard curve.

 


Examples of Data


 


Sandwich ELISA Schematic

  1. A multi-well ELISA assay plate is coated with a known quantity of capture antibody.
  2. Non-specific binding sites on the surface are blocked using a blocking reagent.
  3. The Antigen containing sample is added to the plate, and any antigen present in the sample binds to capture antibody.
  4. The plate is washed to remove any unbound antigen.
  5. A specific detection antibody is added, and binds to antigen.
  6. Enzyme-conjugated detection reagent complex is added, and binds to detection antibody.
  7. Substrate is added, and is converted by enzyme into a color that is measured to determine the quantity of an antigen.

 

 sandwich elisa schematic

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