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Monoclonal Antibody Production - Mouse

NCI Collaboration
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Number:
MAB-001
Size:
1 Each
Price:
Price On Request
Availability:
Ships within 2 weeks.

Monoclonal Antibody Production - Mouse Properties

Description
MONOCLONAL ANTIBODY PRODUCTION (Steps 1-3) Immunization of up to 5 Balb/c mice with protein or peptide antigen, Screening and Evaluation - MAB-001
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Wet Ice
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Monoclonal Antibody Production - Mouse Description

Background
Step 1. Immunization - Immunization of 5 Balb/c mice. In general we begin with a priming dose of 50 µg to 200 µg followed by several boosting doses each containing 50 µg to 200 µg. This regime will continue until sufficient antiserum titer is achieved. Step 2. Screening - Test bleeds are evaluated by using an indirect ELISA. Optimal ELISA conditions are developed to ensure the proper evaluation of future clones. Test bleeds can be sent to your lab for evaluation in other assays. Step 3. Evaluation - Data is evaluated jointly with Rockland staff to determine the performance of antiserum. Antiserum titers and specific assay performance must be acceptable to continue the project.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-50

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry