TrueBlot® For Immunoprecipitation, Western Blot and IP Western Blot

TrueBlot® is a secondary antibody which enables unhindered detection of molecules. Simply substitute your conventional HRP or fluorescent reagent with mouse, rabbit, goat or sheep TrueBlot for unparalleled clarity in results and high quality imaging suitable for reproduction.


Immunoprecipitation protocols and Western Blots provide highly specific results, yet often suffer from heavy/light chain blotting, contamination, and ongoing interference. TrueBlot® products solve nearly all of these problems through increased sensitivity, less background noise, and enhanced accuracy.


 TrueBlot® reagents enable you to generate clear, best-quality data in individual Immunoprecipitation and Western Blots (Nfkb Western Blots as well as GFP Immunoprecipitation Western Blots), but can also be used when the two protocols are combined. Available in several options, from IP Agarose Beads alone, to complete IP/Western Blot kits from goat, mouse, rabbit or sheep.

Learn more about how TrueBlot works!






Mouse TrueBlot® Products




TrueBlot Mouse IP Western Blot Comparison

Mouse TrueBlot®: Anti-Mouse Ig 
Mouse TrueBlot® ULTRA: Anti-Mouse 
Mouse TrueBlot® Set (with IP Beads)  88-7788-31
Mouse TrueBlot® Western Blot Kit 88-8887-31 



Rabbit TrueBlot® Products


TrueBlot Rabbit IP Western Blot Comparison

Rabbit TrueBlot®: Anti-Rabbit
Rabbit TrueBlot® Set (with IP beads) 88-1688-31
Rabbit TrueBlot® Western Blot Kit 88-8886-31




Goat TrueBlot® Products


TrueBlot Goat IP Western Blot Comparison

Goat TrueBlot®: Anti-Goat IgG HRP 18-8814-31
Goat TrueBlot® Set (with IP Agarose beads) 88-1488-31
Goat TrueBlot® Western Blot Kit 88-8884-31



Sheep TrueBlot® Products



Sheep TrueBlot®: Anti-Sheep IgG HRP





TrueBlot® IP Agarose Beads


TrueBlot® Anti-Goat Ig IP Agarose Beads


TrueBlot® Anti-Mouse Ig IP Agarose Beads


TrueBlot® Anti-Rabbit Ig IP Agarose Beads




 New TrueBlot® Products



TrueBlot® IP and Western Blot Kit for Epitope Tags

The TrueBlot® Immunoprecipitation and Western Blot Kit for Epitope Tags contain the critical supporting reagents, buffers, and substrates for IP and WB of the appropriate tagged proteins. Using TrueBlot will allow easy enrichment and clear monitoring of proteins.


Flag TrueBlot


88-8887-382 6X His TrueBlot Kit


88-8887-215 GFP TrueBlot Kit

TrueBlot® IP and Western Blot Kit for DYKDDDDK (FLAG®) Epitope Tag



TrueBlot® IP and Western Blot Kit for 6X HIS Epitope Tag



TrueBlot® IP and Western Blot Kit for GFP Epitope Tag





TrueBlot® Magnetic Beads



TrueBlot® Magnetic Beads are uniform, non-aggregating, super-paramagnetic beads consisting of a ferric oxide core, functionalized with various silane groups to which the affinity groups (e.g. protein A, protein G, anti-mouse IgG, Nickel and others) are firmly attached. The beads are specifically designed, tested and quality controlled for sample preparation, or capturing and purifying targets such as proteins, antigens, antibodies, DNA/RNA, cells and more. TrueBlot® Magnetic Bead design enables faster binding kinetics with high sensitivity and selectivity in both manual and automated biomedical and research applications.




TrueBlot®: Clear Detection of Blotted Target Bands


Rockland’s TrueBlot® product line enables researchers to generate publication-quality western blot data for Nfkb IP Western Blots, Phospho IP Western Blots, and GFP IP Western Blots. Simply substitute your conventional HRP blotting reagent with Goat TrueBlot®, Mouse TrueBlot®, Rabbit TrueBlot® or Sheep TrueBlot®. TrueBlot® is a horseradish peroxidase conjugated immunoblotting detection reagent, which enables unhindered detection of blotted target bands. This results in unparalleled clarity in results and high quality imaging suitable for reproduction.


TrueBlot® preferentially detects the native disulfide form of mouse IgG or rabbit IgG, making it ideal for IP and Western Blot protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot® reduces interference by the ~55 kDa heavy and ~23 kDa light chains of the immunoprecipitating antibody in IP/immunoblotting applications, making Phospho Immunoprecipitation Western Blot data even more accurate. TrueBlot® is also ideal for studying post-translational protein modifications, including phosphorylation, or protein-protein interactions.


For more information or to request a quote for a specific application, contact us today.


Additional Resources

FLAG™ is a registered trademark of Sigma-Aldrich Co. LLC


TrueBlot References Side Bar Button


Fluorescent TrueBlot Side Bar Image



TrueBlot IP Western Blot Technology


TrueBlot Sell Sheet Button


Download the TrueBlot®   IP Western Blotting Reagents PDF 





TrueBlot Protocol Cover


IP / Western Blot Protocol TrueBlot®