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TrueBlot® IP / Western Blot

TrueBlot® is a secondary antibody which enables unhindered detection of molecules. Simply substitute your conventional HRP or fluorescent reagent with mouse, rabbit, goat or sheep TrueBlot for unparalleled clarity in results and high quality imaging suitable for reproduction.

Immunoprecipitation and Western Blots often suffer from heavy/light chain blotting, contamination, and ongoing interference.  TrueBlot products solve these problems through increased sensitivity, less background noise, and enhanced accuracy.

2015 NEWS: TrueBlot regents were originally designed for IP and Western Blot protocols, but have also been proven successful in ELISA, Immunohistochemistry and Immunofluorescence.





Watch the NEW TrueBlot How-To Video!




Trueblot reagents


TrueBlot complete kits


TrueBlot IP Beads


How TrueBlot Works

TrueBlot preferentially detects the native disulfide form of mouse, rabbit, goat, and sheep IgG, making it ideal for an array of protocols involving immunoblotting quantification of immunoprecipitated proteins and detection of target molecules. TrueBlot is highly specific and reduces interference by the ~55 kDa heavy and ~23 kDa light chains of the immunoprecipitating antibody in IP/immunoblotting applications, making your data even more explicit. TrueBlot is also ideal for studying post-translational protein modifications, including phosphorylation, or protein-protein interactions. 
Click here to learn more about how TrueBlot works.



How TrueBlot Works
Click here to view the TrueBlot protocol.





Complete Kits for Immunoprecipitation and Western Blotting


The TrueBlot Immunoprecipitation and Western Blot Kit for Epitope Tags contain the critical supporting reagents, buffers, and substrates for IP and WB of the appropriate tagged proteins. Using TrueBlot will allow easy enrichment and clear monitoring of proteins.



Flag TrueBlot


88-8887-382 6X His TrueBlot Kit


88-8887-215 GFP TrueBlot Kit


TrueBlot IP and Western Blot Kit for



TrueBlot IP and Western Blot Kit for
6X HIS Epitope Tag



TrueBlot IP and Western Blot Kit for 
GFP Epitope Tag



TrueBlot Western Blot Kits


Fluorescent TrueBlot 


Rockland is proud to present a new family of products to our customers. Fluorescent TrueBlot, a new format of our popular TrueBlot product line, combines the power and specificity of the original products with the versatility of fluorescent and near infra-red dyes. Conjugating highly optimized TrueBlot reagents to a full spectrum of fluorescent labels, results in multipurpose reporter molecules that can be used in a variety of immunoassays.


Click here to learn more about Fluorescent TrueBlot.


 18-0217-32 Fluorescent TrueBlot a Mouse IgG Fluorescein WB

Fluorescent TrueBlot Anti-Mouse IgG Fluorescein



Traditional TrueBlot


Rockland’s TrueBlot product line enables researchers to generate publication-quality western blot data for Nfkb IP Western Blots, Phospho IP Western Blots, and GFP IP Western Blots. Simply substitute your conventional HRP blotting reagent with Goat TrueBlot,  Mouse TrueBlot, Rabbit TrueBlot or Sheep TrueBlot. TrueBlot is a horseradish peroxidase conjugated immunoblotting detection reagent, which enables unhindered detection of blotted target bands. This results in unparalleled clarity in results and high quality imaging suitable for reproduction.


TrueBlot Mouse Western Blot Comparison

Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP






IP Beads


TrueBlot IP Beads are a suspension of activated agarose beads coupled to anti-species specific IgG. They are suitable for precipitation of mouse, rabbit, or goat IgGs that are used as the primary antibodies in immunoprecipitation assays. TrueBlot IP Beads are highly specific, and have demonstrated a much greater binding affinity when compared to experiments which used protein A or protein G. TrueBlot IP Beads are available as individual products, and as components of TrueBlot Sets and TrueBlot IP and Western Blot Kits for Epitope Tags.


View all IP Beads  



Click here to view more information on TrueBlot.




Additional Resources

FLAG™ is a registered trademark of Sigma-Aldrich Co. LLC