Molecular biology products and services represent an invaluable tool for the discovery and development
of tools and products in the life sciences and medical field. Rockland’s
growing suite of molecular biology custom products and services will help accelerate
your studies whether working on basic research or performing advanced
translational studies. By directly interacting with our molecular biology
scientists at every step of the process, you gain flexibility and reliability during
the experimental design and execution of your project. Whether your needs
are a simple nucleic acid purification or sequencing project, or assistance
with design of a Chimeric Antigen Receptor (CAR) for a CAR-T
project, Rockland’s custom molecular biology services
team is ready to help. Our Molecular Biology Services include:
Oligonucleotides, gene fragments, even entire genes and
plasmids, can be synthesized according to your custom sequence. Nucleic
acids can also be fully sequenced per your specific requirements. Synthesized
DNA fragments or provided DNA fragments can be cloned into plasmids or other
forms of DNA.
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Native and recombinant proteins can be purified from various sources.
Recombinant gene constructions can be generated and produced in the expression
system of your choice.
Let us do your protein expression or send us the source material and the
target protein will be thoroughly purified from contaminating proteins using a
multi-step process that includes affinity chromatography.
A cDNA library is generated in one of several available vectors using as little as 20 ng of mRNA.
Genomic DNA or mRNA is isolated from either frozen tissue or tissue culture cell preparations.
The clustered regularly
interspaced short palindromic repeats (CRISPR) is derived from a naturally
occurring defense mechanism used by bacteria. The CRISPR/Cas9 system has been
adapted to serve as a versatile platform for RNA-directed genome editing in
mammalian cells. The Cas9 endonuclease can be programed by a dual RNA (crRNA
and tracrRNA), or the core components of these RNAs can also be combined into a
single hybrid guide RNA (gRNA). Target recognition is facilitated by the
presence of a short sequence called a protospacer-adjacent motif (PAM) that
conforms to the sequence NGG. The cleavage of the target DNA by Cas9 triggers two
endogenous repair mechanisms, non-homologous end joining (NHEJ) and
homology-directed repair (HDR). The features of these DNA break repair pathways
can be exploited to generate gene knock-outs or introduce defined modifications
at the site of cleavage.
The CRISPR/Cas9 system has a distinct
advantage over alternative genome editing technologies such as ZFNs and TALENs.
Unlike ZFNs or TALENs, which relies upon the use of customizable DNA-binding
protein nucleases, RNA-based CRISPR provide simplicity and adaptability for
genome editing. Because of this, CRISPR has rapidly become one of the most
popular approaches for genome engineering.
1. CRISPR design and construction
2. Generate knock-in and knockout cell lines of interest
Based on your interest, we can choose lipofection, viral transduction or
nucleofection to optimize CRISPR delivery. The transfected cells will be
selected by antibiotics, MACS or FACS and single clones will be isolated for
3. Gene-editing validation
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Do you have a special molecular biology project but can’t find it on our Molecular Biology Services page? Please contact Customer Support for additional details and let us know how we can help.
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Rockland Immunochemicals Inc.Limerick, PA 19468E-mail: email@example.comPhone: 800.656.7625