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Host Cell Protein (HCP) Antibody Generation

Host Cell Protein Antibody Generation Overview


Biopharmaceuticals require products to be free of host cell protein (HCP) contaminants.  These HCPs may be left behind during the purification process from the expression hosts, such as E. coli, insect, or mammalian cells.   To investigate the presence of residual contamination during the bioprocessing and the final biopharmaceutical product, development of custom polyclonal antibody reagents with maximum coverage against native HCP extracts is required. Please see US FDA Guidelines for Bioanalytical Validation.
Rockland provides multiple customizable options for HCP antibody development.  These options allow flexibility to the researcher to develop early stage through late stage HCP detection reagents. 



 Bioprocessing and Host Cell Protein Reagents


Precision Medicine: Unlocking Biological Mysteries

Rockland and The Scientist brought together a panel of experts to highlight some of the exciting areas where precision medicine is pushing the boundaries of science, technology, and therapy.

Topics covered:
• Tools and techniques for studying genetic variation
• Challenges in data analysis
• Application of genetic information to the development of targeted therapies

With speakers, Dr. Geoffrey Ginsburg, Director of Center for Applied Genomics & Precision Medicine and Dr. Elizabeth Worthey Faculty Investigator and  Director of Bioinformatics.




Bioprocessing Related Products   Catalog #   Applications   Size
Anti-Bovine Serum Albumin (Rabbit) Antibody   201-4133-0025   ELISA, WB, IHC   25 uL
Anti-Bovine Serum Albumin (Mouse) Monoclonal Antibody (BSA)   201-301-033   ELISA, WB, IHC, IP   500 uL
Anti-Streptavidin (Rabbit) Antibody   200-401-095   ELISA, WB, IHC   100 uL
Anti-Biotin (Mouse) Monoclonal Antibody   200-301-098   ELISA, WB, IHC, IF   100 uL
Anti-6X His Epitope Tag (Mouse) Monoclonal Antibody   200-301-382   ELISE, WB, IP   100 ug
Antibody For The Detection Of Flag™ Conjugated Proteins (Mouse) Monoclonal Antibody   200-301-383   ELISA, WB, IHC   100 ug
Anti-Protein A (Goat) Antibody   100-1177   ELISA, WB, IHC   2 mL
Anti-Protein G (Rabbit) Antibody   100-401-138   ELISA, WB   2 mL
Anti-Bovine Igg (H&L) (Rabbit) Antibody   201-4102   ELISA, WB, IHC   50 mg



Host Cell Protein Related Products   Catalog #   Applications   Size
E.Coli Host Cell Protein Control Protein   000-001-J08   ELISA, WB   50 ug
Anti-E.Coli  Host Cell Protein [Total] (Rabbit) Antibody   100-401-J07   ELISA, WB, IHC   100 uL
Anti-E.Coli  High Molecular Weight Fraction Host Cell Protein Antibody   200-401-J05   ELISA, WB   100 ug
Anti-E.Coli Low Molecular Weight Fraction Host Cell Protein Antibody   200-401-J06   ELISA, WB   100 ug
Anti-E.Coli  Host Cell Protein [High And Low Molecular Weight Combined] Antibody   200-401-M61   ELISE, WB   100 ug
Anti-E.Coli  Host Cell Protein Combined Biotin Conjugated Antibody   200-406-M61   ELISA, WB   100 ug
Chemiluminescent Western Blot Kit For Detection Of E.Coli Host Cell Proteins   KCAJ07   WB   1 Kit



Download the Multi-assay Functional Antibodies in HCP Detection poster



HCP Development Poster

Download the Enhanced Standardized HCP poster


Host Cell Protein Workflow


Producing an HCP Antibody: Workflow