Immunohistochemistry Overview


Immunohistochemistry or IHC is a mainstay immunoassay for many laboratories and is used to demonstrate the presence or absence of important proteins, detect post-translational modifications, diagnosis of disease and more. Rockland produces highly active antibodies and conjugates for use in immunohistochemistry experiments.  Rockland also produces high quality reagents for the setup and detection in IHC experiments. Protect your experiments with Rockland antibodies. Compromise elsewhere.



IHC Immunoassays

Immunohistochemistry uses both immunological and biochemical techniques to examine distinct tissue sections by the use of labeled antibodies interacting with target antigens. A visible label or stain is used to visualize the antibody antigen interaction.   IHC allows for the visualization of distinctive cellular components of cells and is frequently used by pathologists and in diagnostics.  Rockland offers Custom Immunohistochemistry Studies for clients that desire that service.

Immunohistochemistry Schematic


IHC sample preparation


The antigen is fixed and adhered to a glass microscope slide.  Formaldehyde fixation is suggested as the routine initial method of choice for tissue and cell fixation, but other methods are also suitable.  Background staining is reduced by with a blocking buffer to block non-specific sites to which the antibodies may bind. Rockland offers common blocking buffers including BSA, normal serum, non-fat dry milk, and an optimized proprietary blocking buffer.  


IHC sample labeling


Labeling is achieved by the reaction of a primary antibody with the immobilized antigen to form an antigen-antibody complex. A second biotinylated antibody specific for the primary antibody host species reacts with the complex. Streptavidin conjugated to the enzyme peroxidase or alkaline phosphatase reacts with this complex. Finally, substrate (view substrates) is added causing a reaction with the enzyme causing colored precipitate to form on the slide at the location of the antigen. The slide is viewed through a standard light microscope and photographed.  A common manipulation in IHC fixing is use of the antigen retrieval technique, a simple method of heating or boiling paraffin-embedded tissue sections to enhance the observed signal.  See Rockland’s IHC experimental protocol.


Immunohistochemistry kits

This MaxTag™ Histo Immunohistochemistry Kit allows for the detection of primary polyclonal or monoclonal antibody provided by the user. This IHC kit relies upon the high-specificity of avidin-biotin binding for visualization of an antigen.  The MaxTag™ Histo Immunohistochemistry Kit is intended to provide a simple, reliable and convenient detection system for immunohistochemical staining.  Complete kit that provides all regents required for immunohistochemistry including antibody enzyme conjugate buffers and substrates, fixative and counterstain.  These simple to use kits guide you through IHC using highly optimized procedures.  The kit components result in low background and high signal producing the most sensitive detection kit available.




Kits for IHC sample preparation and labeling 


Immunohistochemistry Kit: IHC Kit for Human Primary Antibody MaxTag™ Histo  



Immunohistochemistry Kit: IHC Kit for Mouse Primary Antibody MaxTag™ Histo  



Immunohistochemistry Kit: IHC Kit for Rabbit Primary Antibody MaxTag™ Histo



Immunohistochemistry Kit: IHC Kit for Goat Primary Antibody MaxTag™ Histo




View all IHC Kits and Reagents



Mounting, Fixing and Blocking Buffers 



10X PBS MaxTag™ Histo for IHC - MB-0032    MB-0032
PolyMount Mounting Media MaxTag™ Histo for IHC   KHH001
Formaldehyde Fixative 16% (w/v) Ultrapure EM Grade MaxTag™ Histo for IHC   KHF001
Blocking Buffer for Immunohistochemistry - Serum and Azide Free   MB-071-0100
PAP PEN MaxTag™ Histo for IHC   KHP001
  Mounting Fixing Blocking Buffers