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Human PLGF ELISA Kit

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  • Human PLGF Accusignal ELISA Kit
Number:
KOA0292
Size:
1 Kit
Price:
$495.00
Shipping:
$47.00
Handling:
$20.00
Availability:
Ships within 2 weeks.

Human PLGF ELISA Kit Properties

Description
Human PLGF AccuSignal ELISA Kit - KOA0292
Known Cross Reactivity
Human
Application
ELISA : 15.6pg/ml-1000pg/ml
Product Type
Kit
Kit Type
ELISA Kit
Shipping Condition
Wet Ice
AntiCoagulant
Heparin Sodium
Label
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Human PLGF ELISA Kit Description

Background
The protein, also called PGF, is 149 amino acids long and shares 53% identity with the platelet-derived growth factor-like region of human VPF. And the N-glycosylated PLGF protein is secreted into the medium and that it functions as a dimer1. The PLGF gene is mapped to 14q24-q31. There are 3 isoforms of PGF, designated PGF1, PGF2, and PGF3. Only PGF2 is able to bind heparin. Additionally, PGF regulates inter- and intramolecular cross-talk between the VEGF receptor tyrosine kinases FLT1 and FLK12. It also can stimulate angiogenesis and collateral growth in ischemic heart and limb with at least a comparable efficiency to VEGF3.
Synonyms
AI854365, D12S1900, MGC93298, Pgf, PGFL, PIGF, Placenta growth factor, Placental growth factor, Placental growth factor vascular endothelial growth factor related protein, PlGF, PlGF 2, PLGF_HUMAN, PlGF2, SHGC 10760, Vascular endothelial growth factor related protein
Immunogen
Expression system for standard: E.coli; Immunogen sequence: A21-R149
Storage Condition
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
Application Note
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml human PLGF standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA, citrate) or urine to each empty well. It is recommended that each human PLGF standard solution and each sample be measured in duplicate.
Purity/Specifity
Natural and recombinant human PLGF. There is no detectable cross-reactivity with other relevant proteins.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
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Product Type
Molecular Biology Reagents;
Applications
ELISA, WB
Catalog Number
MB-008

Product Type
Molecular Biology Reagents;
Applications
ELISA, WB
Catalog Number
MB-012

Product Type
Molecular Biology Reagents;
Applications
ELISA, WB
Catalog Number
MB-013

Product Type
Molecular Biology Reagents;
Applications
ELISA, WB
Catalog Number
MB-075-1000

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry