Conversion to Recombinant Antibody

Hybridoma or Purified Ab Sequencing

Rockland can convert your monoclonal antibody from a traditional hybridoma into a recombinant antibody format. This can be achieved by gene sequencing from an existing hybridoma cell line, or by de novo protein sequencing from purified antibody preparation. Once the antibody sequence is known, antibody genes in various formats can be synthesized and expressed to obtain recombinant antibody protein that is monoclonal and pure. Recombinant antibodies can also be generated from other discovery projects (i.e., immunization campaigns using various animal species), or selected using antibody display libraries generated from naïve (i.e., non-immunized), hyper-immunized animals, or synthetic antibody libraries.

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Custom Recombinant Monoclonal Antibodies

Rockland's long heritage and expertise in polyclonal & monoclonal antibody discovery and production now includes the conversion of your antibody into recombinant antibody (rAb) formats. By transitioning to a rAb format, the composition, integrity, production yield, quality and performance reproducibility of your antibody can be improved and assured.

rAbs can be produced in a wide range of formats that include common antibody constant regions (e.g., IgG1 Fc, IgG2 Fc, or others) as well as engineered molecular modifications such as fragments of the antibody (e.g., scFv) and inclusion of molecular tags (e.g., FLAG, polyHis, and a range of other options). Various other options include multi-valency and multi-specificity. We can also functionalize your rAbs via genetic fusions to other proteins or biochemically modify them with enzymes (e.g., horse radish peroxidase, alkaline phosphatase, and others), dyes (e.g., FITC and other fluorescent or chemiluminescent molecules) or other tags and molecules (e.g., biotin, PEG).

Whether your antibody is derived from a hybridoma cell line, a yeast or phage display library, or a de novo protein sequencing effort from a purified antibody preparation, we can improve the production and performance of your antibody by conversion to a rAb format.