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Anti-Pdcd4 pS457 (MOUSE) Monoclonal Antibody - 200-301-964
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Anti-Pdcd4 pS457 (RABBIT) Antibody - 600-401-964
Anti-Pdcd4 pS457 (RABBIT) Antibody - 600-401-964
Primary Antibodies  >  Phospho Specific Antibodies
 

Pdcd4 phospho S457 Antibody

Rabbit Polyclonal
Code

600-401-964
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Rockland's affinity purified anti-Pdcd4 pS457 antibody was used at 1.25 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate positive staining of human breast epithelial cells at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
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Synonyms: Death up-regulated gene protein antibody, Dug antibody, H731 antibody, Ma3 antibody, Neoplastic transformation inhibitor antibody, Neoplastic transformation inhibitor protein antibody, Nuclear antigen H731 antibody

Pdcd4 phospho S457 Antibody Properties

Description
Anti-Pdcd4 pS457 (RABBIT) Antibody - 600-401-964
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse, rat, Xenopus
Clonality
Polyclonal
Application
ELISA : 1:400,000
Western Blot : 1:500 - 1:2,000
Immunochemistry: 21.25 - 2.5 µg/ml
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
PDCD4
Download CofA
Lot#  

Pdcd4 phospho S457 Antibody Description

Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Programmed cell death 4 (Pdcd4) is a novel tumor supressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding Ser457 in the human Pdcd4 protein.
Immunogen Type
Peptide
Post Translational Modification
Phosphorylation
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user. By western blot, a band approximately 52 kDa in size corresponding to Pdcd4 protein is expected in the appropriate cell lysate or extract.
Purity/Specifity
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against nonphospho-peptide. This antibody is specific for human Pdcd4 protein phosphorylated at Ser457.  A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence.  Cross-reactivity with Pdcd4 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
Tzu-Hsuan Huang, GB Loeb, R Hsu, A Heidersbach, A Brincat, D Horiuchi, RJ Lebbink, YY Mo, A Goga, MT McManus. Up-regulation of miR-21 by HER2/neu Signaling Promotes Cell Invasion J. Biol. Chem. 2009 284: 18515-18524. First Published on May 6, 2009, doi:10.1074/jbc.M109.006676
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat, xenopus
Catalog Number
200-301-964

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat, xenopus
Catalog Number
200-301-964S

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat, xenopod
Catalog Number
200-301-B17

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat, Xenopus
Catalog Number
600-401-964

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry