Primary Antibodies  >  Glucose Energy Metabolism Antibodies
 

xCT Antibody

Rabbit Polyclonal
NCI Collaboration JCYIA Product
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Number:
600-401-GU3
Size:
100 µg
Price:
$339.00
Shipping:
$41.00
Handling:
$20.00
Availability:
Ships next business day.

xCT Antibody Properties

Description
Anti-xCT (RABBIT) Antibody - 600-401-GU3
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 5.0 ug/ml
IF Microscopy : 10 ug/ml
Flow Cytometry : 1:200
Western Blot : 1:1000
Immunohistochemistry: 1:200
ImmunoPrecipitation: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
IgG
Label
Concentration
1.02 mg/ml by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
SLC7A11
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xCT Antibody Description

Background
xCT belongs to the amino acid-polyamine-organocation (APC) superfamily and L-type amino acid transporter (LAT) family. xCT gene encodes a member of a heteromeric, sodium-independent, anionic amino acid transport system that is highly specific for cysteine and glutamate. In this system, designated Xc(-), the anionic form of cysteine is transported in exchange for glutamate. Increased expression of this gene results in neuronal cell death. xCT may be associated with kaposi sarcoma, dyscalculia, cystinuria, spondylolsis, and anemia of prematurity. Anti-xCT Antibody is useful for researchers interested in Cancer Research, Glucose/Energy Metabolism Research, and Cell Surface Protein Interaction Research.
Synonyms
rabbit anti-xCT antibody, SLC7A11, Cystine/glutamate transporter, Amino acid transport system xc-, Calcium channel blocker resistance protein CCBR1, Solute carrier family 7 member 11
Immunogen
Anti-xCT antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human xCT conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-xCT Antibody has been tested in Western Blot, ELISA, Immunofluorescence and Flow cytometry. Expect a band at 55.4 kDa in western blot using appropriate lysates. Positive control used NIH-3T3, Hela, HCT-116, A549, or PC3 whole cell lysates at 0.1-1.0µg/mL for WB and PC3 with MeOH at 10µg/mL in IF. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.
Purity/Specifity
This affinity purified antibody is directed against human xCT. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with human and orangutan, and 88% identity to mouse.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
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Product Type
Cell Lysates;
Applications
WB
Catalog Number
W09-000-364

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-073

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry